| 產(chǎn)品編號 | bs-7149R-Bio |
| 英文名稱 | Rabbit Anti-ZNF127/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的鋅指蛋白127抗體 |
| 別 名 | D15S9; Makorin ring finger protein 3; Mkrn3; MKRN3_HUMAN; Probable E3 ubiquitin-protein ligase makorin-3; RING finger protein 63; RNF63; ZFP127; Zinc finger protein 127; ZNF127. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Pig, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF127 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. This gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping this gene. [provided by RefSeq, Jul 2008] Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Tissue Specificity: Expressed in testis, brain, heart and kidney. Ubiquitously detected at low levels throughout the entire embryo, but expression is highest in the ventricular layers of the brain. DISEASE: Expressed at the blastocyst stage and the embryonic days 8-17, as well as in undifferentiated and differentiated embryonic stem cells. Expressed in the arcuate nucleus of both male and female animals. Levels of expression are highest on postnatal days 10 and 12, begin to decline on day 15, and reaches a nadir by days 18 to 22, at which time expression is 10 to 20% of the levels detected at 10 days. The timing of the decline in protein expression correlated with the ages at which arcuate KISS1 and TAC2 have been shown to increase, heralding the onset of puberty. Similarity: Contains 3 C3H1-type zinc fingers. Contains 1 RING-type zinc finger. Database links: Entrez Gene: 7681 Human Entrez Gene: 22652 Mouse Omim: 603856 Human SwissProt: Q13064 Human SwissProt: Q60764 Mouse Unigene: 679587 Human Unigene: 72964 Human Unigene: 146 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
