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MTHFD1 Recombinant Mouse mAb (bsm-60464M)  
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產(chǎn)品編號 bsm-60464M
英文名稱 MTHFD1 Recombinant Mouse mAb
中文名稱 亞甲基四氫葉酸脫氫酶單克隆抗體
別    名 C1TC_HUMAN; C-1-tetrahydrofolate synthase, cytoplasmic; MTHFC; MTHFD; C1-THF synthase; Epididymis secretory sperm binding protein; C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed; Methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1; CIMAH;  
研究領域 腫瘤  細胞生物  信號轉導  新陳代謝  
抗體來源 Mouse
克隆類型 Recombinant
克 隆 號 F9D9
交叉反應 Human (predicted: Mouse)
產(chǎn)品應用 WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 110 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG1, Kappa
純化方法 affinity purified by Protein G
緩 沖 液 PBS, Glycerol, BSA.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous.

Database links:

Entrez Gene: 4522 Human

Entrez Gene: 108156 Mouse

Entrez Gene: 64300 Rat

Omim: 172460 Human

SwissProt: P11586 Human

SwissProt: Q922D8 Mouse

SwissProt: P27653 Rat



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: room temperature 2h Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 102 kDa Observed MW: 102 kDa
Cell line: HEK-293 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60464M
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