| 產(chǎn)品編號 | bsm-51745M |
| 英文名稱 | SULT2B1 Mouse mAb |
| 中文名稱 | SULT2B1單克隆抗體 |
| 別 名 | Alcohol sulfotransferase; EC 2.8.2.2; HSST2; Hydroxysteroid sulfotransferase 2; ST2B1; ST2B1_HUMAN; Sulfotransferase 2B1; Sulfotransferase family cytosolic 2B member 1; SULT2B1a; SULT2B1b. |
| 研究領域 | 心血管 信號轉(zhuǎn)導 生長因子和激素 脂蛋白 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | C11G7 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SULT2B1. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] Function: Catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung. Similarity: Belongs to the sulfotransferase 1 family. SWISS: O00204 Gene ID: 6820 Database links: Entrez Gene: 6820 Human SwissProt: O00204 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: MCF-7 cell lysates
Lane 2: MDA-MB-468 cell lysates
Primary: Anti-SULT2B1 (bsm-51745M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
Sample:
Lane 1: MCF-7 cell lysates
Primary: Anti-SULT2B1 (bsm-51745M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
