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Cell Counting Kit-8 (BA00208)  
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產(chǎn)品編號 BA00208
英文名稱 Cell Counting Kit-8
中文名稱 細胞增殖及毒性檢測試劑盒
別    名 Cell Counting Kit-8 (CCK-8); CCK-8; WST-8; Enhanced Cell Counting Kit-8; CCK-8 Cell Proliferation and Cytotoxicity Assay Kit; CCK-8 WST-8; Cell Counting Kit - 8;   CCK-8細胞增殖及毒性檢測試劑盒; CCK8試劑盒; CCK-8/WST-8試劑盒; CCK-8細胞活力檢測試劑盒; 細胞計數(shù)試劑盒-8(CCK-8); CCK-8試劑盒(細胞增殖及毒性檢測試劑盒); Cell Counting Kit-8(CCK-8試劑盒); 細胞計數(shù)試劑盒-8; Cell Counting Kit-8(CCK-8)細胞活性檢測試劑盒; 細胞活性檢測試劑盒;
Specific References  (99)     |     BA00208 has been referenced in 99 publications.
[IF=17.694] Hu, Jinyuan. et al. Design of synthetic collagens that assemble into supramolecular banded fibers as a functional biomaterial testbed. NAT COMMUN. 2022 Nov;13(1):1-13  
[IF=10.75] Liu, Zhenni. et al. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. INT J BIOL SCI. 2023 Jan;19(3):950-966  
[IF=10.684] Peng Luo. et al. OP3-4 peptide sustained-release hydrogel inhibits osteoclast formation and promotes vascularization to promote bone regeneration in a rat femoral defect model. BIOENG TRANSL MED. 2022 Oct;:e10414  Other ;  Other.  
[IF=9.685] Yang, Wenjing. et al. BTN3A1 promotes tumor progression and radiation resistance in esophageal squamous cell carcinoma by regulating ULK1-mediated autophagy. CELL DEATH DIS. 2022 Nov;13(11):1-17  
[IF=9.417] Zhenjia Che. et al. Bifunctionalized hydrogels promote angiogenesis and osseointegration at the interface of three-dimensionally printed porous titanium scaffolds. MATER DESIGN. 2022 Nov;223:111118  Other ;  Other.  
[IF=9.229] Mengjia Wang. et al. Binding Peptide-Promoted Biofunctionalization of Graphene Paper with Hydroxyapatite for Stimulating Osteogenic Differentiation of Mesenchymal Stem Cells. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX  
[IF=8.786] Junjun Guo. et al. TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. FRONT IMMUNOL. 2023; 14: 1114620  
[IF=8.786] Xiaogang Shen. et al. Integrating machine learning and single-cell trajectories to analyze T-cell exhaustion to predict prognosis and immunotherapy in colon cancer patients. FRONT IMMUNOL. 2023; 14: 1162843  
[IF=8.025] Gaofeng Cai. et al. Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice. INT J BIOL MACROMOL. 2023 Jan;:123386  
[IF=8.013] Yuchen Liu. et al. Docosahexaenoic Acid Attenuates Radiation-Induced Myocardial Fibrosis by Inhibiting the p38/ET-1 Pathway in Cardiomyocytes. INT J RADIAT ONCOL. 2022 Dec;:  
[IF=7.666] Shulipan Mulati. et al. 6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer. CELLS-BASEL. 2022 Jan;11(22):3678  
[IF=7.419] Li-Ping Yu. et al. In vivo identification of the pharmacodynamic ingredients of Polygonum cuspidatum for remedying the mitochondria to alleviate metabolic dysfunction–associated fatty liver disease. BIOMED PHARMACOTHER. 2022 Dec;156:113849  Other ;  Other.  
[IF=7.397] Shunjie Yu. et al. TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome. IMMUNOLOGY. 2022 May 03  
[IF=7.109] Zhao, Renchang. et al. Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes. CELL DEATH DISCOV. 2023 Jan;9(1):1-10  
[IF=6.656] Gaofeng Cai. et al. The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. PHYTOMEDICINE. 2022 Aug;103:154232  
[IF=6.656] Zhenwei Zhou. et al. BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis. PHYTOMEDICINE. 2023 May;113:154742  
[IF=6.58] Luqin Wan. et al. The advanced glycation end-products (AGEs)/ROS/NLRP3 inflammasome axis contributes to delayed diabetic corneal wound healing and nerve regeneration. Int J Biol Sci. 2022; 18(2): 809–825  Other ;  Other.  
[IF=6.53] Shilei Zhang. et al. A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue. BIOMED PHARMACOTHER. 2022 Jun;150:113004  Other ;  Other.  
[IF=6.53] Xing-Hua Xiao. et al. Magnolol alleviates hypoxia-induced pulmonary vascular remodeling through inhibition of phenotypic transformation in pulmonary arterial smooth muscle cells. BIOMED PHARMACOTHER. 2022 Jun;150:113060  
[IF=6.529] Huifeng Wang. et al. Periplocin ameliorates mouse age-related meibomian gland dysfunction through up-regulation of Na/K-ATPase via SRC pathway. Biomed Pharmacother. 2022 Feb;146:112487  
[IF=6.388] Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 Apr;:  
[IF=6.306] Yu Han. et al. Structural characterization and transcript-metabolite correlation network of immunostimulatory effects of sulfated polysaccharides from green alga Ulva pertusa. Food Chem. 2021 Apr;342:128537  
[IF=6.289] Zhengqing Zhu. et al. Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration. Mater Design. 2021 Oct;208:109882  Other ;  
[IF=6.208] Lan Wang. et al. Fenbendazole Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice via Suppression of Fibroblast-to-Myofibroblast Differentiation. INT J MOL SCI. 2022 Jan;23(22):14088  
[IF=6.117] Xiaoshan Liang. et al. Folic Acid Ameliorates Synaptic Impairment following Cerebral Ischemia/Reperfusion Injury via Inhibiting Excessive Activation of NMDA Receptors. J NUTR BIOCHEM. 2022 Nov;:109209  
[IF=6.081] Min Yu. et al. RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. Biomedicines. 2021 Sep;9(9):1244  Other ;  mouse.  
[IF=6.081] Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936  
[IF=6.023] Huan Liu. et al. Effect of DEHP and DnOP on mitochondrial damage and related pathways of Nrf2 and SIRT1/PGC-1α in HepG2 cells. Food Chem Toxicol. 2021 Dec;158:112696  CCK8 ;  
[IF=5.923] Jiali Xiong. et al. Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation. Int J Mol Sci. 2022 Jan;23(3):1413  Other ;  Other.  
[IF=5.846] Mengqi Gu. et al. Influence of placental exosomes from early onset preeclampsia women umbilical cord plasma on human umbilical vein endothelial cells. FRONT CARDIOVASC MED. 2022 Dec 23;9:1061340  
保存條件 Protect from light. Store at 2-8℃ for one year. Store at -20℃ for two year.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Cell Counting Kit-8,即增強型 CCK-8 試劑盒,是一種基于 WST-8 而廣泛應(yīng)用于細胞增殖和細胞毒性的快速、高靈敏度、無放射性的比色檢測試劑盒。WST-8 在電子耦合試劑存在的情況下,可以被線粒體內(nèi)的一些脫氫酶還原生成橙黃色的 formazan,且細胞增殖越多越快,則顏色越深;細胞毒性越大,則顏色越淺。對于同樣的細胞,顏色的深淺 (生成的 formazan 量) 和細胞數(shù)目呈線性關(guān)系。

WST-8 是 MTT 的一種升級替代產(chǎn)品,和 MTT 或其它 MTT 類似產(chǎn)品,如 XTT、MTS 等相比有明顯的優(yōu)點。第一,MTT 被線粒體內(nèi)的一些脫氫酶還原生成的 formazan 不是水溶性的,需要有特定的溶劑來溶解;而 WST-8 和 XTT、MTS 產(chǎn)生的 formazan 都是水溶性的,可以省去后續(xù)的溶解步驟。第二,WST-8 產(chǎn)生的 formazan 比 XTT 和 MTS 產(chǎn)生的 formazan 更易溶解。第三,WST-8 比 XTT 和 MTS更加穩(wěn)定,使實驗結(jié)果更可靠。第四,WST-8 和 MTT、XTT 等相比線性范圍更寬,靈敏度更高,并且更加穩(wěn)定。

WST-8 對細胞無明顯毒性,可以直接加入到細胞樣品中,無需預配各種成分;顯色后,可以在不同時間反復用酶標儀讀板,檢測時間更加靈活,便于確定最佳測定時間;酚紅和血清對本試劑盒的測定無明顯影響。

產(chǎn)品圖片
Bioss CCK-8產(chǎn)品(BA00208)與國際G品牌對比圖:圖中左側(cè)樣本為SP2/O的檢測數(shù)據(jù),右側(cè)樣本為3T3的檢測數(shù)據(jù).Bioss產(chǎn)品穩(wěn)定性與特異性與國際G品牌同等優(yōu)質(zhì).
Bioss CCK-8產(chǎn)品(BA00208)與國際D品牌對比圖:圖中左側(cè)樣本為SP2/O的檢測數(shù)據(jù),右側(cè)樣本為3T3的檢測數(shù)據(jù).Bioss產(chǎn)品穩(wěn)定性與特異性與國際D品牌同等優(yōu)質(zhì).
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