產品編號 | C01-03001 |
英文名稱 | Normal Goat Serum (10%) |
中文名稱 | 10%封閉山羊血清 |
別 名 | 10% Normal Goat Serum; Blocking Buffer for Immunol Staining; Normal Goat Serum Blocking Buffer; blocking goat serum; blocking grade goat serum; 10% NGS, goat serum blocking buffer; 免疫染色封閉液; 免疫組化封閉液; 免疫組化染色封閉液; HIC封閉液; 10%山羊血清; 10% 山羊血清; 10%山羊血清封閉液; 正常山羊血清; |
Specific References (16) | C01-03001 has been referenced in 16 publications.
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保存條件 | Store at 2-8℃. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產品介紹 |
Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications. Application: Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding. 封閉血清適用于免疫組化或者免疫熒光實驗中樣本的封閉。可以封閉待檢樣本中與蛋白質非特異性結合的位點,另外還可以封閉樣品中內源性的Fc片段結合位點,阻斷抗體與樣品中的Fc受體結合,從而降低背景,減少假陽性。在選擇封閉血清時,要注意封閉用血清中不能含有目標蛋白,且與一抗來源不同,可用與二抗相同來源的封閉血清。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |