產(chǎn)品編號 | C05-07001 |
英文名稱 | Western Antibody Diluent (TBS) |
中文名稱 | 通用型WB抗體稀釋液(TBS) |
別 名 | Western Primary Antibody Diluent; Antibody Diluent; Primary Antibody Dilution Buffer for Western Blot; Western Primary Antibody Dilution Buffer; Antibody Diluent(TBST); Western一抗稀釋液; Western一抗稀釋緩沖液; Western抗體稀釋液; WB抗體稀釋液; Western blotting 專用一抗二抗稀釋液; 通用型WB抗體稀釋液 |
Specific References (3) | C05-07001 has been referenced in 3 publications.
[IF=6.953] Chao Xin. et al. The novel nanocomplexes containing deoxycholic acid-grafted chitosan and oleanolic acid displays the hepatoprotective effect against CCl4-induced liver injury in vivo. Int J Biol Macromol. 2021 Aug;185:338 WB ;
[IF=4.501] Fang Shi. et al. Phosphatidylinositol 3-Kinase/Protein Kinase B/Mammalian Target of the Rapamycin Pathway-Related Protein Expression in Lung Squamous Cell Carcinoma and Its Correlation with Lymph Node Metastasis.. J ONCOL. 2022 Aug;2022:4537256-4537256 Other ; Other.
[IF=3.115] Yongfang Ou. et al. Dietary Ochratoxin A Contamination Modulates Oxidative Stress, Inflammation Processes and Causes Fibrosis in in vitro and in vivo Lung Models. FRONT BIOSCI-LANDMRK. 2023 Feb;28(2):22
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保存條件 | 2-8℃保存,一年有效。. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品介紹 |
產(chǎn)品簡介: 通用型WB抗體稀釋液(Western Antibody Dilution)適用于免疫印跡實驗中一抗二抗的稀釋。本產(chǎn)品添加了抗體保護劑、穩(wěn)定劑和防腐劑等成分,能有效減少一抗的非特異性結(jié)合,增加了稀釋后抗體的穩(wěn)定性。用此稀釋液稀釋后的一抗二抗可在2-8℃環(huán)境下穩(wěn)定一周。 使用方法: 參考一抗二抗的使用說明,以及樣品中目的蛋白的含量或個人優(yōu)化條件,按照適當比例將適量抗體加入抗體稀釋液中,充分混勻后即可使用??贵w最好以濃縮液形式保存,現(xiàn)用現(xiàn)稀釋,稀釋后的抗體可2-8℃保存建議不要超過一周。 注意事項: 1.如需抗體回收再利用,建議在2-8℃保存,以用于下次的 Western實驗。進行抗體的結(jié)合反應時可適當補充抗體,建議盡量在 4℃進行一抗和蛋白膜的結(jié)合反應,以減緩一抗的衰減速度。 2.如果發(fā)現(xiàn)有沉淀或細菌污染請棄用。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |