| 產品編號 | bsm-33004M |
| 英文名稱 | His tag Mouse mAb |
| 中文名稱 | 聚組氨酸抗體標簽單克隆抗體 |
| 別 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
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Specific References (1) | bsm-33004M has been referenced in 1 publications.
[IF=4.961] Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524 WB ; Human.
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| 產品類型 | 標簽抗體 |
| 研究領域 | 細胞生物 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6000000 |
| 交叉反應 | Recombinant protein |
| 產品應用 | WB=1:5000-50000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated HHHHHH peptide |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 蛋白質純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白 1） 6xHis與不同的Sepharose純化填料結合（捆綁），可不受構象的影響，在天然或變形的條件下可進行進一步純化。 2） 可在溫和的洗脫液環(huán)境中，其結合、洗滌及洗脫步驟不會對蛋白結構產生影響。 3） 6xHis標簽比普通（Tag）要小得多，經純化后的蛋白可直接進行下游操作?？捎糜谌魏伪磉_系統(tǒng)（包括： 細菌，桿狀病毒和哺乳動物） 4） 6xHis標簽在生理溶液pH下不帶電荷，其標簽不會干涉重組蛋白的結構和功能， 6xHis不會影響蛋白分泌。 5） 6xHis標簽免疫原性差，重組蛋白可以不必除去標簽而直接作為抗原進行免疫。 |
| 產品圖片 |
Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
50 ng Recombinant GFP protein (bs-33009P) per lane probed with His tag monoclonal antibody respectively, unconjugated (bsm-33004M) at 1:5000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug
Primary: Anti-His tag (bsm-33004M) at 1/2000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
