| 產(chǎn)品編號(hào) | bs-0220P |
| 英文名稱 | Alkaline Phosphatase(≥5,500 U/mg) |
| 中文名稱 | 堿性磷酸酶 (進(jìn)口分裝 ≥5,500 U/mg)蛋白 |
| 別 名 | Alkaline Phosphatase from calf intestine; Phosphatase, Alkaline from bovine intestinal mucosa; Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP-1; ALP; ALPP; ALPPL2; ALPPL; PALP; PLAP-1; PLAP; |
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Specific References (1) | bs-0220P has been referenced in 1 publications.
[IF=3.864] Xie W? et al. A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.Analyst. 2018 Jun 11;143(12):2901-2907. other ;
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| CAS | |
| 理論分子量 | ~160 kDa |
| 檢測分子量 | |
| 保存條件 | Stored at 2-8℃. Do not freeze. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Alkaline phosphatase is highly purified from calf intestine. Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays. CAS：9001-78-9 分子量：dimer ~160 kDa MDL：MFCD00131849 濃度：≥10 mg/mL 活性：≥5500 DEA單位/mg蛋白質(zhì) 單位定義：一個(gè)DEA單位將在37°C的pH 9.8下每分鐘水解1μmol 4-硝基苯磷酸酯（一個(gè)甘氨酸單位相當(dāng)于~3個(gè)DEA單位） 性狀：溶液（含5 mM Tris、5 mM MgCl2和0.1 mM ZnCl2的50%甘油溶液，pH 7.0） 用途：堿性磷酸酶可以用來去磷酸酪蛋白和其他蛋白質(zhì)。堿性磷酸酶也可以用來去磷酸化DNA或RNA的5′端，以防止自連接。用堿性磷酸酶去磷酸化后，DNA或RNA也可以用放射性標(biāo)記的磷酸鹽（通過T4多核苷酸激酶）標(biāo)記。 推薦用于抗體和蛋白質(zhì)結(jié)合的高比活性等級。 |
