| 產(chǎn)品編號 | bs-10075R |
| 英文名稱 | Nidogen2 Rabbit pAb |
| 中文名稱 | 骨巢蛋白抗體 |
| 別 名 | NID 2; NID2; Nidogen2; Nidogen 2; Nidogen-2; Osteonidogen; NID2_HUMAN. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉導 細胞周期蛋白 細胞粘附分子 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 148 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nidogen2: 501-600/1375 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nidogen 2 is an adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell extracellular matrix interactions. Function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. Subunit: Interacts with LAMA2. Interacts with COL13A1. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Tissue Specificity: Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. Post-translational modifications: Highly N- and O-glycosylated. Similarity: Contains 5 EGF-like domains. Contains 5 LDL-receptor class B repeats. Contains 1 NIDO domain. Contains 1 nidogen G2 beta-barrel domain. Contains 2 thyroglobulin type-1 domains. SWISS: Q14112 Gene ID: 22795 Database links: Entrez Gene: 22795 Human Entrez Gene: 18074 Mouse Omim: 605399 Human SwissProt: Q14112 Human SwissProt: O88322 Mouse Unigene: 147697 Human Unigene: 369840 Human Unigene: 20348 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
