| 產(chǎn)品編號 | bs-0296G-Cy5 |
| 英文名稱 | Goat Anti-Mouse IgG H&L antibody |
| 中文名稱 | Cy5標記的羊抗小鼠IgG H&L |
| 別 名 | Goat Anti-Mouse IgG H&L (Cy5); Immunoglobulin G; |
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Specific References (5) | bs-0296G-Cy5 has been referenced in 5 publications.
[IF=9.995] Huijuan Tan. et al. Phase separation of SGS3 drives siRNA body formation and promotes endogenous gene silencing. CELL REP. 2023 Jan;42:111985 IF ; Arabidopsis seedlings,yeast.
[IF=5.869] Qianqian Guo . et al. Heterologous prime-boost immunization co-targeting dual antigens inhibit tumor growth and relapse. Oncoimmunology. 2020;9(1):1841392 ICC ; Hamster.
[IF=5.215] Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243 FCM ; Human.
[IF=4.486] Shen L et al. FPS-ZM1 Alleviates Neuroinflammation in Focal Cerebral Ischemia Rats via Blocking Ligand/RAGE/DIAPH1 PathwayACS Chem Neurosci.2021 Jan 6;12(1):63-78. IF ;
[IF=4.021] Jiang X et al. Overexpression of augmenter of liver regeneration (ALR) mitigates the effect of H 2 O 2-induced endoplasmic reticulum stress in renal tubule epithelial cells. Apoptosis.2019. ICF ; Mouse.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse |
| 產(chǎn)品應用 | Flow-Cyt=1:100-1000,IF=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 2.0 mg/ml |
| 免 疫 原 | Native Mouse IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-cy5) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
