| 產(chǎn)品編號 | bs-6030R |
| 英文名稱 | CD200 Rabbit pAb |
| 中文名稱 | 膜糖蛋白CD200抗體 |
| 別 名 | CD200 antigen; CD200 molecule; MOX1; MOX2; MRC; MRC OX 2 antigen; My033; OX 2; OX 2 membrane glycoprotein precursor; OX-2 membrane glycoprotein; OX2G; OX2G_HUMAN. |
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Specific References (2) | bs-6030R has been referenced in 2 publications.
[IF=2.8] Clark, David A., et al. "The Receptor for the CD200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts." American Journal of Reproductive Immunology 74.5 (2015): 387-391. IHC-P ; Human.
[IF=2.8] Clark, David A., Jorge L. Arredondo, and Sukhbinder Dhesy-Thind. "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks’ gestation." Journal of Reproductive Immunology 112 (2015): 20-23. IHC-P ; Human.
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| 研究領(lǐng)域 | 細胞生物 免疫學 細胞表面分子 糖蛋白 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | 40-50 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD200: 41-140/278 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Function: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. Subunit: Interacts with CD200R1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P41217 Gene ID: 4345 Database links: Entrez Gene: 4345 Human Omim: 155970 Human SwissProt: P41217 Human Unigene: 79015 Human CD200是Ⅰ型膜糖蛋白，為免疫球蛋白超家族的新成員，廣泛表達在人類胸腺細胞、B細胞、激活的T細胞、濾泡狀樹突細胞、神經(jīng)元及血管內(nèi)皮上?？杀磉_于B細胞及其增殖性疾病和一部分T細胞。近年來研究發(fā)現(xiàn)，CD200與CD200R間的相互作用可以傳遞抑制性信號減低髓樣細胞的活性，改變其遷移。CD200在免疫疾病和抑制排斥反應(yīng)中作為一種免疫耐受信號，它的一些相關(guān)分子在免疫調(diào)節(jié)中也具有一定的作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
