| 產(chǎn)品編號 | bs-4236R |
| 英文名稱 | ADAM17 Rabbit pAb |
| 中文名稱 | 腫瘤壞死因子α轉(zhuǎn)換酶抗體 |
| 別 名 | A disintegrin and metalloproteinase domain 17; ADAM 17; ADAM metallopeptidase domain 17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; MGC71942; Snake venom like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; Tumor Necrosis Factor alpha Converting Enzyme; A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme); ADA17_HUMAN. |
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Specific References (6) | bs-4236R has been referenced in 6 publications.
[IF=6.961] Chan Yen-Ju. et al. Blue light exposure collapses the inner blood-retinal barrier by accelerating endothelial CLDN5 degradation through the disturbance of GNAZ and the activation of ADAM17. FLUIDS BARRIERS CNS. 2023 Dec;20(1):1-16 WB ; Human,Mouse.
[IF=4.757] Maria Consiglia Trotta. et al. Oral Administration of Vitamin D3 Prevents Corneal Damage in a Knock-Out Mouse Model of Sjögren’s Syndrome. BIOMEDICINES. 2023 Feb;11(2):616 IF ; Mouse.
[IF=4.379] Xiao, Wenyuan. et al. Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis. Sci Rep-Uk. 2022 Mar;12(1):1-14 IF ; Mouse.
[IF=3.449] Haolu Sun. et al. iRhom1 rescues cognitive dysfunction in multiple sclerosis via preventing myelin injury. Genes Brain Behav. 2021 Nov;20(8):e12771 WB ; Mouse.
[IF=2.08] Li, Qing, et al. "Identification of ADAM10 and ADAM17 with potential roles in the spermatogenesis of the Chinese mitten crab, Eriocheir sinensis." Gene(2015). WB ;
[IF=0] Cyro José de Almeida Guardiola et al. DC-STAMP and TACE Levels are Higher in Patients with Periodontitis. Braz Dent J
. Mar-Apr 2020;31(2):122-126. WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68/91 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM17: 201-300/824 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]. Subunit: Interacts with MAD2L1, MAPK14 and MUC1. Subcellular Location: Membrane. Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. Similarity: Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. SWISS: P78536 Gene ID: 6868 Database links: Entrez Gene: 6868 Human Entrez Gene: 11491 Mouse Omim: 603639 Human SwissProt: P78536 Human SwissProt: Q9Z0F8 Mouse Unigene: 404914 Human Unigene: 27681 Mouse Unigene: 144585 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ADAM17 polyclonal antibody, unconjugated (bs-4236R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (ADAM17) polyclonal Antibody, unconjugated (bs-4236R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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