| 產(chǎn)品編號 | bs-2385R |
| 英文名稱 | Kinesin 5B Rabbit pAb |
| 中文名稱 | 驅(qū)動蛋白KIF5B抗體 |
| 別 名 | Kinesin 5B; Kinesin5B; Kinesin-5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; KLF5_HUMAN. |
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Specific References (1) | bs-2385R has been referenced in 1 publications.
[IF=3.53] Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D (2015) Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE 10(7): e0134390. IHC-F ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLF5: 61-160/457 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq, Jul 2008] Function: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Subunit: Interacts with WWP1. Subcellular Location: Nucleus. Tissue Specificity: Expressed only in testis and placenta. Post-translational modifications: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: Q13887 Gene ID: 688 Database links: Entrez Gene: 688 Human Entrez Gene: 12224 Mouse Omim: 602903 Human SwissProt: Q13887 Human SwissProt: Q9Z0Z7 Mouse Unigene: 508234 Human Unigene: 30262 Mouse Unigene: 8954 Rat |
| 產(chǎn)品圖片 |
Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-Kinesin 5B (Bs-2385R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (bs-2385R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (bs-2385R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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