| 產(chǎn)品編號 | bs-3882R |
| 英文名稱 | Rabbit Anti-GPX1 antibody |
| 中文名稱 | 谷胱甘肽過氧化酶1 |
| 別 名 | Glutathione peroxidase 1; Cellular glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GSHPx-1; GSHPX1; MGC14399; MGC88245. |
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Specific References (11) | bs-3882R has been referenced in 11 publications.
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| 研究領域 | 腫瘤 心血管 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat,Sheep (predicted: Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Glutathione Peroxidase 1: 51-150/201 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene product belongs to the family of glutathione peroxidase, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq]. Subunit: Homotetramer. Interacts with MIEN1 (By similarity). Subcellular Location: Cytoplasm. Similarity: Belongs to the glutathione peroxidase family. SWISS: P11352 Gene ID: 2876 Database links: Entrez Gene: 461015 Chimpanzee Entrez Gene: 2876 Human Entrez Gene: 706732 Macaque Monkey Entrez Gene: 14775 Mouse Entrez Gene: 100438450 Orangutan Omim: 138320 Human SwissProt: Q0EFA0 Chimpanzee SwissProt: P07203 Human SwissProt: Q865R2 Macaque Monkey SwissProt: P11352 Mouse SwissProt: Q4AEI3 Orangutan Unigene: 76686 Human Unigene: 1090 Mouse Unigene: 11323 Rat 保護紅細胞中血紅蛋白的氧化分解. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Rat Liver tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Kidney tissue lysates
Primary: Anti-GPX1 (bs-3882R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- GPX1 (bs-3882R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-GPX1 antibody (bs-3882R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
