| 產(chǎn)品編號(hào) | bs-3165R |
| 英文名稱 | Phospho-Gab1 (Tyr627) Rabbit pAb |
| 中文名稱 | 磷酸化接頭蛋白Gab 1抗體 |
| 別 名 | GAB1(phospho Y627); Gab1(phospho-Tyr627); p-Gab1(Tyr627); GAB1_HUMAN; GRB2-associated-binding protein 1; GAB1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1; |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Gab1 around the phosphorylation site of Tyr627: VE(p-Y)LD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Function: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Subunit: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2A, GRB2, GAB1, PIK3R1 and SOS1. Post-translational modifications: Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Similarity: Belongs to the GAB family. Contains 1 PH domain. SWISS: Q13480 Gene ID: 2549 Database links: Entrez Gene: 2549 Human Entrez Gene: 14388 Mouse SwissProt: Q13480 Human SwissProt: Q9QYY0 Mouse Gab1作為一種分布廣泛的接頭蛋白，幾乎能連接所有類型的受體（如酪氨酸激酶受體、G-蛋白偶聯(lián)受體、細(xì)胞因子與抗原受體等，Gab1蛋白屬于接頭蛋白Gab家族,該家族蛋白因能與生長因子受體結(jié)合蛋白2（Grb2）相結(jié)合而得名。作為接頭蛋白,Gab1蛋白能被多種受體酪氨酸激酶或非受體酪氨酸激酶激活,接受胞外多種生長因子、細(xì)胞因子和一些T/B細(xì)胞抗原受體的刺激,介導(dǎo)PI3K/Akt和Ras/MAPK等多條信號(hào)轉(zhuǎn)導(dǎo)途徑,具有促進(jìn)細(xì)胞生長、遷移、調(diào)節(jié)免疫等多種生物學(xué)功能,與糖尿病、腫瘤、心血管疾病等的發(fā)生發(fā)展密切相關(guān)。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-Gab1 (Tyr627) polyclonal antibody, unconjugated (bs-3165R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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