| 產(chǎn)品編號 | bs-3099R |
| 英文名稱 | Phospho-Cofilin (Ser3) Rabbit pAb |
| 中文名稱 | 磷酸化絲切蛋白(Ser3)抗體 |
| 別 名 | Cofilin(phospho-Ser3); Cofilin(phospho S3); 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. |
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Specific References (3) | bs-3099R has been referenced in 3 publications.
[IF=4.014] Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051 WB ; Chicken.
[IF=2.894] Wufuer R et al. Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapyPhotodiagnosis Photodyn Ther.2020 Dec 8;102143. WB ; Human.
[IF=2.705] Xue Y et al. Methamphetamine?reduces?expressions?of?tight?junction?proteins,?rearranges?F-actincytoskeleton?and?increases?the?blood?brain?barrier?permeability?via?the?RhoA/ROCK-dependent pathway. Biochem Biophys Res Commun. 2019 Feb 5;509(2):395-401. WB ; Rat.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 細(xì)胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3: A(p-S)GV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]. Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Subunit: Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods. Subcellular Location: Nucleus matrix. Cytoplasm, cytoskeleton. Tissue Specificity: Widely distributed in various tissues. Post-translational modifications: Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Similarity: Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain. SWISS: P23528 Gene ID: 1072 Database links: Entrez Gene: 1072 Human Entrez Gene: 12631 Mouse Entrez Gene: 494432 Sheep Omim: 601442 Human SwissProt: P23528 Human SwissProt: P18760 Mouse SwissProt: Q6B7M7 Sheep Unigene: 52428 Cow Unigene: 170622 Human Unigene: 329655 Mouse Unigene: 11675 Rat Unigene: 13212 Sheep 絲切蛋白(cofilin)是細(xì)胞骨架塑形的重要調(diào)節(jié)因素,其主要功能是分解肌動蛋白微絲和增加肌動蛋白單體從肌動蛋白微絲的末端解離的速度,從而促進(jìn)肌動蛋白微絲的循環(huán),是目前為止已發(fā)現(xiàn)的肌動蛋白結(jié)合蛋白家族中唯一能夠改變肌動蛋白微絲扭曲結(jié)構(gòu)的蛋白. |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Cofilin (Ser3) (bs-3099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (bs-3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (bs-3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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