| 產(chǎn)品編號 | bs-1743R |
| 英文名稱 | Dopamine D3 receptor Rabbit pAb |
| 中文名稱 | 多巴胺受體D3抗體 |
| 別 名 | DRD3; 3dopamine receptor; D; Dopamine receptor D3; DRD3; Dopamine receptor 3; ETM1; FET1; D(3) dopamine receptor; DRD3; DRD3_HUMAN. |
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Specific References (6) | bs-1743R has been referenced in 6 publications.
[IF=6.05] Andrew Kisling. et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro. Front Cardiovasc Med. 2021; 8: 732282 IF ; Mouse.
[IF=5.108] Yang K et al. Synaptic dopamine release is positively regulated by SNAP-25 that involves in benzo [a] pyrene-induced neurotoxicity. Chemosphere. 2019 Jul 15;237:124378. IHC-P,ICF&WB ; Rat.
[IF=4.831] Shi L et al. Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinson's disease through AKT/mTOR pathway. Aging (Albany NY)
. 2020 May 18;12(10):9515-9533. WB ; Monkey.
[IF=4.432] Ting Li. et al. Rotigotine-loaded microspheres exerts the antinociceptive effect via central dopaminergic system. Eur J Pharmacol. 2021 Nov;910:174443 WB ; Rat.
[IF=4.181] Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446. WB ; Mouse.
[IF=2.35] Xu, Jiao-jiao, et al. "Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells." Acta Pharmacologica Sinica (2014). WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse DRD3: 352-446/446 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is expressed in phylogenetically older regions of the brain, suggesting that it plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Also known as: dopamine D3 receptor; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1. Subunit: Interacts with CLIC6. Interacts with GRK4. Interacts with PALM. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. Tissue Specificity: Brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P30728 Gene ID: 13490 Database links: Entrez Gene: 1814 Human Entrez Gene: 13490 Mouse Omim: 126451 Human SwissProt: P35462 Human SwissProt: P30728 Mouse Unigene: 121478 Human Unigene: 439735 Mouse Unigene: 10356 Rat 多巴胺受體D3常與某些精神、神經(jīng)疾病有關(guān)，如精神分裂癥、早發(fā)性癡呆等。但最新研究表明多巴胺受體與腫瘤細(xì)胞多藥耐藥有關(guān)。 |
| 產(chǎn)品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Sample:
HepG2 Cell Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.
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