| 產品編號 | bs-0999R |
| 英文名稱 | Rabbit Anti-GM-CSF antibody |
| 中文名稱 | 粒細胞-巨噬細胞克隆刺激因子抗體 |
| 別 名 | Granulocyte-Macrophage Colony Stimulating Factor; Burst Promoting Activity; CMCSF; Colony Stimulating Factor 2; Colony stimulating factor; CSF 2; CSF Alpha; CSF; CSF2; Eosinophil Colony Stimulating Factor; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; MGC131935; MGC138897; Molgramostin; Pluripoietin Alpha; Sargramostim; granulocyte-macrophage colony-stimulating factor precursor; CSF2_HUMAN; CSF2_MOUSE. |
| 研究領域 | 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Pig (predicted: Mouse,Rat,Recombinant protein) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse GM-CSF: 71-141/141 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]. Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Subunit: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Subcellular Location: Secreted. Similarity: Belongs to the GM-CSF family. SWISS: P01587 Gene ID: 12981 Database links: Entrez Gene: 12981 Mouse SwissProt: P01587 Mouse Unigene: 4922 Mouse GM-CSF是血管內皮細胞、單核細胞和成纖維細胞合成的糖蛋白。能促進中性粒細胞成熟；刺激成熟的粒細胞從骨髓釋出；增強中性粒細胞趨化及吞噬功能。 |
| 產品圖片 |
Sample:
Lane 1: Recombined Trx/His-tag porcine?GM-CSF protein (Aa18-144) at 5 ug
Lane 2: Recombined?His-tag?porcine?GM-CSF protein
Primary: Anti-GM-CSF (bs-0999R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/15 kD
Observed band size: 33/15 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
