| 產(chǎn)品編號 | bs-1201R |
| 英文名稱 | PAX8 Rabbit pAb |
| 中文名稱 | 配對盒基因8抗體 |
| 別 名 | Paired Box Gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired Domain Gene 8; PAX 8; PAX-8; PAX8_HUMAN. |
 |
Specific References (5) | bs-1201R has been referenced in 5 publications.
[IF=4.872] Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats 2.5Ecotoxicol Environ Saf.2021 Jan 15;208:111720. IHC、WB ; Rat.
[IF=4.711] Xiuzhen Du. et al. Membranous and nuclear staining of CLDN18 in HPV-independent and HPV-associated endocervical adenocarcinomas. CANCER MED-US. 2022 Jul;: IHC ; Human.
[IF=4.223] Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13 WB,IHC ; Rat.
[IF=2.34] Huang et al. Upregulation of TSHR, TTF-1, and PAX8 in Nodular Goiter Is Associated with Iodine Deficiency in the Follicular Lumen. (2016) Int.J.Endocrinol. 2016:2492450 IHC,WB ; Human.
[IF=1.38] Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014). Human.
|
| 研究領(lǐng)域 | 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Sheep,Cow,Dog,Goat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAX8: 21-130/457 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells. Function: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Subunit: Interacts with WWTR1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in the excretory system, thyroid gland and Wilms tumors. DISEASE: Hypothyroidism, congenital, non-goitrous, 2 (CHNG2) [MIM:218700]: A disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 paired domain. SWISS: Q06710 Gene ID: 7849 Database links: Entrez Gene: 7849 Human Entrez Gene: 18510 Mouse Omim: 167415 Human SwissProt: Q06710 Human SwissProt: Q00288 Mouse Unigene: 469728 Human Unigene: 2533 Mouse Unigene: 10392 Rat 同源結(jié)構(gòu)域蛋白（Homeodomain Proteins） PAX8屬于轉(zhuǎn)錄抑制因子PAXs的一種。在胚胎發(fā)育和腫瘤生長中起關(guān)鍵作用。其突變和某些腫瘤的發(fā)病有關(guān)。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated(bs-1201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PAX8) polyclonal Antibody, Unconjugated (bs-1201R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Cell:HT-29;
Concentration:1:100
Host/Isotype:Rabbit/IgG(blue line)
Flow cytometric analysis of Rabbit IgG isotype control on HT-29(blue) compared with primary antibody(Cat#: bs-1021R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Primary Antibody:Rabbit Anti-TLR4 antibody(bs-1021R, green line);
Isotype Control Antibody: Rabbit IgG (orange line);
Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE (white blue line)
Positive control: Jurkat cells(blue line)
Protocol
1.Jurkat cells were washed twice with phosphate-buffered saline (PBS).
2. An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
