| 產(chǎn)品編號 | bs-1003R |
| 英文名稱 | Rabbit Anti-EAAT1 antibody |
| 中文名稱 | 膠質(zhì)細胞谷氨酸運載蛋白1抗體 |
| 別 名 | EA6; EAAT1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST1; Glial high affinity glutamate transporter; High affinity neuronal glutamate transporter; Slc1a3; Sodium dependent glutamate/aspartate transporter; EAA1_HUMAN. |
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Specific References (4) | bs-1003R has been referenced in 4 publications.
[IF=4.879] Tomasz St?pień. et al. Neuropathological Changes in the Brains of Suicide Killers. Biomolecules. 2021 Nov;11(11):1674 IHC ; Human.
[IF=4.721] Ohmichi T et al.Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases.(2018)Parkinsonism Relat Disord. Nov 20. ELISA ; Human.
[IF=1.06] Xiao, Ke-Qing, et al. "Effect of subarachnoid nerve block anesthesia on glutamate transporter GLAST and GLT-1 expressions in rabbits." Asian Pacific Journal of Tropical Medicine (2015). IHC-P ; Rabbit.
[IF=0.66] Lu, Jun, et al. "Expression of GFAP, GLAST and GLT-1 in astrocytes in patients with mesial temporal lobe epilepsy." Journal of Hainan Medical University 22.2 (2016): 87-90. IHC-P ; Human.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EAAT1: 301-400/512 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014] Function: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Subcellular Location: Membrane; Multi-pass membrane protein Tissue Specificity: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Post-translational modifications: Glycosylated. DISEASE: Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. SWISS: P43003 Gene ID: 6507 Database links: Entrez Gene: 6507 Human Entrez Gene: 20512 Mouse Omim: 600111 Human SwissProt: P43003 Human SwissProt: P56564 Mouse Unigene: 481918 Human Unigene: 204834 Mouse Unigene: 34134 Rat EAAT-1主要用于神經(jīng)膠質(zhì)細胞的損傷及退變方面的研究. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-EAAT1 (bs-1003R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Tissue/cell: rat brain tissue(Glial cells); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-EAAT1 antibody (bs-1003R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
