產(chǎn)品編號 | bs-0292R |
英文名稱 | Rabbit Anti-Bovine Serum Albumin antibody |
中文名稱 | 牛血清白蛋白抗體 |
別 名 | ALB; Albumin; Allergen Bos d 6; BSA; Serum albumin; ALBU_BOVIN. |
Specific References (6) | bs-0292R has been referenced in 6 publications.
[IF=21.567] Yutaro Mori. et al. KIM-1 mediates fatty acid uptake by renal tubular cells to promote progressive diabetic kidney disease. Cell Metab. 2021 May;33:1042 IF ; Human, Mouse.
[IF=4.774] Yasunobu Asawa. et al. Carborane as an Alternative Efficient Hydrophobic Tag for Protein Degradation. Bioconjugate Chem. 2021;XXXX(XXX):XXX-XXX WB ; Human.
[IF=3.73] Chen, Cheng-Hsien, et al. "MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis." PloS one 9.6 (2014): e99802. other ; Bovine.
[IF=3.057] Wei Zhang. et al. Nanometer-Sized Boron Loaded Liposomes Containing Fe3O4 Magnetic Nanoparticles and Tributyl Borate and Anti-Albumin from Bovine Serum Antibody for Thermal Neutron Detection. Materials. 2021 Jan;14(11):3040
[IF=2.408] Jingru Qin. et al. In-silico analysis identifies the anti-liver injury targets of diammonium glycyrrhizinate: validated in perfluorooctanoic acid-lesioned mouse model. 2022 Mar 31 IHC ; Mouse.
[IF=2.2] Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950. WB ; Bovine.
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研究領域 | 心血管 免疫學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human,Mouse,Rat,Cow,Goat |
產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 69kDa |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Bovine Serum Albumin: full length |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]. Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Subcellular Location: Secreted. Tissue Specificity: Plasma. Post-translational modifications: Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylation sites are present in the extracelllular medium. Acetylated on Lys-223 by acetylsalicylic acid. DISEASE: Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P02769 Gene ID: 280717 Database links:
Entrez Gene: 213 Human Entrez Gene: 11657 Mouse Omim: 103600 Human SwissProt: P02768 Human SwissProt: P07724 Mouse
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產(chǎn)品圖片 |
Sample:
BSA (Cow) at 40 ug
Primary: Anti-Bovine Serum Albumin (bs-0292R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Sample:
Serum (Cow) at 40 ug
Primary: Anti-Bovine Serum Albumin (bs-0292R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Sample:
Lane 1: Serum (Human) at 20 ug
Lane 2: Serum (Mouse) at 20 ug
Lane 3: Serum (Rat) at 20 ug
Lane 4: Serum (Goat) at 20 ug
Lane 5: Serum (Cow) at 20 ug
Primary: Anti-Bovine Serum Albumin (bs-0292R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |