| 產品編號 | bsm-62671R |
| 英文名稱 | TRIM24 Recombinant Rabbit mAb |
| 中文名稱 | 轉錄中介因子Tif1α重組兔單抗 |
| 別 名 | hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA; Trim24. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 117 kDa |
| 檢測分子量 | 130 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TRIM24: 405-455 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). SWISS: O15164 Gene ID: 8805 與許多核受體和輔活化子相互作用并調節(jié)靶基因轉錄的轉錄輔活化子。依賴于組蛋白H3修飾與染色質相互作用，對組蛋白H3具有最高親和力，組蛋白H3在“Lys-4”（H3K4me0）處未修飾并且在“Lys-23”（H3K23ac）處乙?；?。具有E3蛋白泛素連接酶活性，促進p53/TP53泛素化和蛋白酶體降解。在細胞增殖和凋亡的調節(jié)中發(fā)揮作用，至少部分通過其對p53/TP53水平的影響。通過AR、GCR/NR3C1、甲狀腺激素受體（TR）和ESR1上調配體依賴性轉錄激活。通過維甲酸（RA）受體調節(jié)轉錄激活，包括RAR |
| 產品圖片 |
Western blot analysis of HeLa cell lysate. Using TRIM24 (bsm-62671R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
